Production, Characterization and Kinetic Studies of Proteases by Screening of Indigenous Biomass

Authors

  • Sidra Chughtai Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Khizar Hayat Bhatti Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Abeer Zahra Department of Chemistry, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Ejaz Hussain Siddique Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Muhammad Zeeshan Baqir Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Kashwar Syed Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujarat-50700, Pakistan
  • Tanveer Hussain Department of Botany, Mirpur University of Science and Technology, Mirpur-10250, AJK, Pakistan
  • Muhammad Ishtiaq Department of Botany, Mirpur University of Science and Technology, Mirpur-10250, AJK, Pakistan
  • Muhammad Ajaib Department of Botany, Mirpur University of Science and Technology, Mirpur-10250, AJK, Paki

Keywords:

Aspergillus niger, Corn Cobs, Purification, Response Surface Methodology, Soilid State Fermentation

Abstract

Proteases are vital industrial biocatalysts that form the largest group of enzymes acting as proteinases, peptidases, and amidases. Solid State fermentation (SSF) is recognized as an effective method to produce protease enzyme. The enzyme activity profile was obtained by performing enzyme assay showed that corn cobs gave maximum protease activity i.e., 53.297U/mL after seven days of incubation. Response surface methodology (RSM) was used to study the effect of different parameters on enzyme production. From the results inferred by RSM, the maximum enzyme activity was observed at 50°C, at pH 8.0 inoculated with 2mL inoculum size after seven days of incubation. KM and Vmax kinetic parameters were calculated 0.5219 mM and 12.19512 mM/min. The unique properties of protease enzyme such as high specificity, biodegradability and decreased the generation of waste materials makes it important enzyme for biotechnological applications.

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Published

2023-07-05

How to Cite

Sidra Chughtai, Khizar Hayat Bhatti, Abeer Zahra, Ejaz Hussain Siddique, Muhammad Zeeshan Baqir, Kashwar Syed, Tanveer Hussain, Muhammad Ishtiaq, & Muhammad Ajaib. (2023). Production, Characterization and Kinetic Studies of Proteases by Screening of Indigenous Biomass. GU Journal of Phytosciences, 3(3), 217–223. Retrieved from https://jphytosci.com/index.php/GUJP/article/view/123

Issue

Vol. 3 No. 3 (2023): GU Journal of Phytosciences [July, 2023]

Section

Articles

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